2P2R
Crystal structure of the third KH domain of human Poly(C)-Binding Protein-2 in complex with C-rich strand of human telomeric DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-28 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.11588, 0.97945 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 81.069, 81.069, 87.815 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 54.800 - 1.600 |
| R-factor | 0.2066 |
| Rwork | 0.205 |
| R-free | 0.24225 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.349 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.800 | 1.642 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.033 | 0.406 |
| Number of reflections | 15516 | |
| <I/σ(I)> | 22.8 | 7.3 |
| Completeness [%] | 98.5 | 84.66 |
| Redundancy | 6.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.18 | 295 | 2M ammonium sulfate, 80 mM lithium sulfate, 100 mM CAPS, pH 8.18, 5% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | ammonium sulfate | ||
| 2 | 1 | 1 | lithium sulfate | ||
| 3 | 1 | 1 | CAPS | ||
| 4 | 1 | 1 | glycerol | ||
| 5 | 1 | 1 | water | ||
| 6 | 1 | 2 | ammonium sulfate | ||
| 7 | 1 | 2 | lithium sulfate | ||
| 8 | 1 | 2 | CAPS | ||
| 9 | 1 | 2 | glycerol |
