2P2R
Crystal structure of the third KH domain of human Poly(C)-Binding Protein-2 in complex with C-rich strand of human telomeric DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-06-28 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.11588, 0.97945 |
Spacegroup name | H 3 2 |
Unit cell lengths | 81.069, 81.069, 87.815 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 54.800 - 1.600 |
R-factor | 0.2066 |
Rwork | 0.205 |
R-free | 0.24225 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.349 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.800 | 1.642 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.033 | 0.406 |
Number of reflections | 15516 | |
<I/σ(I)> | 22.8 | 7.3 |
Completeness [%] | 98.5 | 84.66 |
Redundancy | 6.8 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.18 | 295 | 2M ammonium sulfate, 80 mM lithium sulfate, 100 mM CAPS, pH 8.18, 5% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | ammonium sulfate | ||
2 | 1 | 1 | lithium sulfate | ||
3 | 1 | 1 | CAPS | ||
4 | 1 | 1 | glycerol | ||
5 | 1 | 1 | water | ||
6 | 1 | 2 | ammonium sulfate | ||
7 | 1 | 2 | lithium sulfate | ||
8 | 1 | 2 | CAPS | ||
9 | 1 | 2 | glycerol |