2P23
Crystal structure of human FGF19
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-27 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97928 |
| Spacegroup name | P 3 |
| Unit cell lengths | 67.297, 67.297, 54.557 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 1.800 |
| Rwork | 0.251 |
| R-free | 0.28500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pwa |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.302 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 25585 | |
| <I/σ(I)> | 25.6 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4.6 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.75 | 298 | 100 mM tri-sodium citrate, 14% PEG 1000, pH 5.75, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
