2P1W
structure of the phosphothreonine lyase SpvC, the effector protein from Salmonella
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BSRF BEAMLINE 3W1A |
Synchrotron site | BSRF |
Beamline | 3W1A |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2007-01-23 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 0.9794 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 70.352, 70.352, 101.243 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.300 |
Rwork | 0.191 |
R-free | 0.22900 |
Structure solution method | SAD |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE (2.12) |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.098 | 0.361 |
Number of reflections | 15052 | |
<I/σ(I)> | 8.4 | 30 |
Completeness [%] | 97.2 | 72.2 |
Redundancy | 27.3 | 23.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 12% PEG3350, 0.1M TrisHCl pH8.0, 5% Tacsimate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |