2P1Q
Mechanism of Auxin Perception by the TIR1 ubiquitin ligase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-09-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 101.207, 82.472, 125.053 |
Unit cell angles | 90.00, 104.05, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.910 |
R-factor | 0.17386 |
Rwork | 0.172 |
R-free | 0.21333 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | : 2P1M |
RMSD bond length | 0.017 |
RMSD bond angle | 1.634 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.030 |
High resolution limit [Å] | 1.910 | 1.910 |
Number of reflections | 69793 | |
<I/σ(I)> | 33 | 4.9 |
Completeness [%] | 99.9 | 100 |
Redundancy | 3.8 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 100 mM BTP, 10% 14% PEG 20,000, 200 mM NaCl, and 5 mM DTT, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |