2P1Q
Mechanism of Auxin Perception by the TIR1 ubiquitin ligase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 101.207, 82.472, 125.053 |
| Unit cell angles | 90.00, 104.05, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.910 |
| R-factor | 0.17386 |
| Rwork | 0.172 |
| R-free | 0.21333 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | : 2P1M |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.634 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Number of reflections | 69793 | |
| <I/σ(I)> | 33 | 4.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 100 mM BTP, 10% 14% PEG 20,000, 200 mM NaCl, and 5 mM DTT, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
