2P1I
Plasmodium yoelii Ribonucleotide Reductase Subunit R2 (PY03671)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-23 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97972 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 192.040, 153.460, 143.360 |
| Unit cell angles | 90.00, 132.00, 90.00 |
Refinement procedure
| Resolution | 19.990 - 2.700 |
| R-factor | 0.30692 |
| Rwork | 0.304 |
| R-free | 0.36797 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1piy |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.004 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.070 | 0.767 |
| Number of reflections | 104627 | |
| <I/σ(I)> | 14.7 | 1.2 |
| Completeness [%] | 98.7 | 88 |
| Redundancy | 3.6 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 2.0 M Sodium formate, 100 mM Hepes, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
