2P1G
Crystal structure of a putative xylanase from Bacteroides fragilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.645, 65.830, 74.061 |
| Unit cell angles | 90.00, 103.84, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.169 |
| Rwork | 0.167 |
| R-free | 0.21400 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.282 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.011 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.157 | 0.368 |
| Number of reflections | 44349 | |
| <I/σ(I)> | 10.1 | 3.9 |
| Completeness [%] | 99.1 | 98.4 |
| Redundancy | 5.3 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 294 | 100mM Bis-Tris pH 6.5, 28% PEG MME 2000, VAPOR DIFFUSION, temperature 294K |
