2P19
Crystal structure of bacterial regulatory protein of gntR family from Corynebacterium glutamicum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97980 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 106.418, 106.418, 225.950 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.170 - 2.100 |
| R-factor | 0.17991 |
| Rwork | 0.178 |
| R-free | 0.21874 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.321 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.158 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.104 | 0.542 |
| Number of reflections | 42209 | |
| <I/σ(I)> | 35.77 | 4.58 |
| Completeness [%] | 99.4 | 97.69 |
| Redundancy | 23.5 | 19.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 1.1M di-Ammonium tartrate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
