2P12
Crystal structure of protein of unknown function DUF402 from Rhodococcus sp. RHA1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97916 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 132.203, 38.980, 70.721 |
| Unit cell angles | 90.00, 107.69, 90.00 |
Refinement procedure
| Resolution | 40.300 - 1.630 |
| R-factor | 0.175 |
| Rwork | 0.171 |
| R-free | 0.21600 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.596 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.300 | 1.690 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.075 | 0.349 |
| Number of reflections | 40684 | |
| <I/σ(I)> | 14.6 | 2.54 |
| Completeness [%] | 93.1 | 52 |
| Redundancy | 7.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 0.2M Ammonium acetate, 0.1M Bis-Tris pH 5.5, 25% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
