2P0Y
Crystal structure of Q88YI3_LACPL from Lactobacillus plantarum. Northeast Structural Genomics Consortium target LpR6
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-08 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97921, 0.97950, 0.96791 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 70.658, 78.130, 149.559 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| Rwork | 0.274 |
| R-free | 0.33900 |
| Structure solution method | MAD |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | RESOLVE (2.08) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.114 | 0.397 |
| Number of reflections | 15950 | |
| <I/σ(I)> | 9.1 | |
| Completeness [%] | 96.2 | 96.6 |
| Redundancy | 4.3 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH UNDER OIL | 278 | 2.0 M Sodium chloride, 10 % PEG 6000, MICROBATCH UNDER OIL, temperature 278K | |
| 1 | MICROBATCH UNDER OIL | 278 | 2.0 M Sodium chloride, 10 % PEG 6000, MICROBATCH UNDER OIL, temperature 278K | |
| 1 | MICROBATCH UNDER OIL | 278 | 2.0 M Sodium chloride, 10 % PEG 6000, MICROBATCH UNDER OIL, temperature 278K |
