2P0O
Crystal structure of a conserved protein from locus EF_2437 in Enterococcus faecalis with an unknown function
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-02-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97935 |
Spacegroup name | P 63 |
Unit cell lengths | 86.244, 86.244, 109.764 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.900 - 2.150 |
R-factor | 0.18543 |
Rwork | 0.183 |
R-free | 0.23345 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.480 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXD |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.900 | 2.230 |
High resolution limit [Å] | 2.150 | 2.150 |
Number of reflections | 23984 | |
<I/σ(I)> | 11.3 | 2.4 |
Completeness [%] | 95.2 | 74 |
Redundancy | 10.5 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.1M Bis-Tris pH 6.5, 25% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 289K |