2P0J
Structure of restriction endonuclease BstYI bound to non-cognate DNA
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2003-12-12 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.979, 0.968 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 86.570, 118.990, 102.240 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.100 |
Rwork | 0.199 |
R-free | 0.23900 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.218 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.100 |
Number of reflections | 31206 |
Redundancy | 7.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 1.4M sodium citrate, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | sodium citrate | ||
2 | 1 | 1 | HEPES | ||
3 | 1 | 1 | H2O | ||
4 | 1 | 2 | sodium citrate | ||
5 | 1 | 2 | HEPES |