2P0G
Crystal structure of Selenoprotein W-related protein from Vibrio cholerae. Northeast Structural Genomics target VcR75
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-02-12 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97912 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 81.602, 81.602, 105.848 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.300 |
Rwork | 0.226 |
R-free | 0.24400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2oka |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | COMO (1.2) |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
Rmerge | 0.100 | 0.050 | 0.386 |
Number of reflections | 34831 | ||
<I/σ(I)> | 8.7 | ||
Completeness [%] | 99.7 | 98.7 | 99.7 |
Redundancy | 6.9 | 7.4 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 0.1M Na Acetate, 8% PEG 4000, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |