2P0F

ArhGAP9 PH domain in complex with Ins(1,3,5)P3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2004-03-18
Detector	ADSC QUANTUM 315
Wavelength(s)	0.90020
Spacegroup name	P 21 21 21
Unit cell lengths	28.751, 36.601, 113.708
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.910
R-factor	0.21028
Rwork	0.209
R-free	0.23014
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dro
RMSD bond length	0.017
RMSD bond angle	1.680
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.000	25.000	1.970
High resolution limit [Å]	1.900	4.090	1.900
Rmerge	0.055	0.029	0.291
Number of reflections	9223
<I/σ(I)>	18.4
Completeness [%]	93.1	93.1	91.3
Redundancy	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	20% PEG4000, 100mM HEPES, 5% glycerol, 5mM DTT, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K