2P0D
ArhGAP9 PH domain in complex with Ins(1,4,5)P3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-D |
Synchrotron site | APS |
Beamline | 14-BM-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-07-29 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9202 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 41.510, 95.110, 29.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.570 - 1.811 |
R-factor | 0.2 |
Rwork | 0.199 |
R-free | 0.22200 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.017 |
RMSD bond angle | 1.982 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.048 | 0.361 |
Number of reflections | 11480 | |
<I/σ(I)> | 17 | |
Completeness [%] | 99.2 | 93.6 |
Redundancy | 12 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 26% PEG4000, 100mM HEPES, 10% glycerol, 5mM DTT, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |