2P08

Structure of the N-terminally truncated PAS domain of signal transduction histidine kinase from Nostoc punctiforme PCC 73102 with homology to the H-NOXA/H-NOBA domain of the soluble guanylyl cyclase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-02-07
Detector	CUSTOM-MADE
Spacegroup name	P 61 2 2
Unit cell lengths	72.332, 72.332, 169.080
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.360 - 2.000
R-factor	0.24056
Rwork	0.239
R-free	0.27802
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.373
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	35.400
High resolution limit [Å]	2.000
Number of reflections	17432
<I/σ(I)>	12.2
Completeness [%]	99.4
Redundancy	5.68

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.7	277	1.7-1.9M NH4SO4, 100mM TrisHCl pH 7.7-9.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K