2OZZ
Crystal structure of YhfZ from Shigella flexneri
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-04-02 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97912 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.270, 62.550, 170.192 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.710 - 2.298 |
R-factor | 0.229 |
Rwork | 0.222 |
R-free | 0.28800 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.570 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.710 | 2.380 |
High resolution limit [Å] | 2.298 | 2.298 |
Rmerge | 0.108 | 0.601 |
Number of reflections | 23138 | |
<I/σ(I)> | 10.1 | 2.21 |
Completeness [%] | 99.2 | 95 |
Redundancy | 14.3 | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 291 | 0.2 M Ammonium sulfate, 0.1 M Sodium citrate, pH 5.6, 20 % PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 291K |