2OZV
Crystal structure of a predicted O-methyltransferase, protein Atu636 from Agrobacterium tumefaciens.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97906, 0.97923 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.544, 78.054, 62.261 |
| Unit cell angles | 90.00, 94.55, 90.00 |
Refinement procedure
| Resolution | 31.030 - 1.700 |
| R-factor | 0.17958 |
| Rwork | 0.178 |
| R-free | 0.21399 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.422 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.030 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 48650 | |
| <I/σ(I)> | 13.2 | 2.4 |
| Completeness [%] | 95.5 | 68.1 |
| Redundancy | 6.2 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Potassium thiocyanate, 30% PEG MME 2000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
