2OZQ
Crystal Structure of apo-MUP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-01-31 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 53.786, 53.786, 137.521 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.370 - 1.800 |
| R-factor | 0.18339 |
| Rwork | 0.181 |
| R-free | 0.23276 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1qy0 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.205 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.400 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.110 | 0.370 |
| Number of reflections | 16532 | |
| <I/σ(I)> | 13.4 | 4 |
| Completeness [%] | 85.9 | 95.8 |
| Redundancy | 5.2 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 55 mM Cadmium Chloride, 0.1 M Malate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
