2OZK
Structure of an N-Terminal Truncated Form of Nendou (NSP15) From SARS-CORONAVIRUS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2006-03-10 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 31 |
| Unit cell lengths | 98.974, 98.974, 214.926 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 85.750 - 2.900 |
| R-factor | 0.24624 |
| Rwork | 0.243 |
| R-free | 0.30068 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2h85 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.485 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.750 | 2.970 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.085 | 0.466 |
| Number of reflections | 52135 | |
| <I/σ(I)> | 14.4 | 1.44 |
| Completeness [%] | 98.1 | 89.9 |
| Redundancy | 2.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 0.2 M sodium bromide, 0.1 M sodium acetate, PH 5.5 and 25% PEG 2000MME, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
