2OZG

Crystal structure of GCN5-related N-acetyltransferase (YP_325469.1) from Anabaena variabilis ATCC 29413 at 2.00 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL1-5
Synchrotron site	SSRL
Beamline	BL1-5
Temperature [K]	100
Detector technology	CCD
Collection date	2007-01-30
Detector	ADSC QUANTUM 4
Wavelength(s)	0.918381, 0.979310, 0.978835
Spacegroup name	H 3 2
Unit cell lengths	174.965, 174.965, 71.888
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.161 - 2.000
R-factor	0.164
Rwork	0.161
R-free	0.21500
Structure solution method	MAD
RMSD bond length	0.018
RMSD bond angle	1.823
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELX
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.161	29.160	2.050
High resolution limit [Å]	2.000	8.940	2.000
Rmerge	0.089	0.037	0.731
Total number of observations		1548	9435
Number of reflections	28396
<I/σ(I)>	6.4	16.1	1.1
Completeness [%]	99.8	96.9	99.6
Redundancy	5.1	4.6	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.3	277	NANODROP, 0.2M K3 Citrate, 20% PEG 3350, No Buffer, pH 8.3, VAPOR DIFFUSION, SITTING DROP, temperature 277K