2OZF
The crystal structure of the 2nd PDZ domain of the human NHERF-1 (SLC9A3R1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.91830 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 43.304, 54.715, 96.724 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.530 - 1.500 |
| R-factor | 0.17239 |
| Rwork | 0.170 |
| R-free | 0.21057 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.801 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.530 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.066 | 0.601 |
| Number of reflections | 18038 | |
| Completeness [%] | 95.8 | 99.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.2M NaNO3, 0.1M BTProp, 20% PEG 3350, 10% Ethylene glycol, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
