2OZE
The Crystal structure of Delta protein of pSM19035 from Streptoccocus pyogenes
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-07-28 |
Detector | MARRESEARCH |
Wavelength(s) | 0.972 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 82.968, 82.968, 233.998 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.500 - 1.830 |
Rwork | 0.192 |
R-free | 0.21804 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bej |
RMSD bond length | 0.013 |
RMSD bond angle | 1.340 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.870 |
High resolution limit [Å] | 1.830 | 1.830 |
Rmerge | 0.062 | 0.396 |
Number of reflections | 43046 | |
<I/σ(I)> | 11.8 | 3.9 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 9.2 | 8.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.9M Na-Hepes, 2% Ethanol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |