2OZB
Structure of a human Prp31-15.5K-U4 snRNA complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-05-03 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.05 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 83.555, 99.896, 169.645 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.600 |
R-factor | 0.20784 |
Rwork | 0.206 |
R-free | 0.24756 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1e7k |
RMSD bond length | 0.011 |
RMSD bond angle | 1.501 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.700 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.107 | 0.663 |
Number of reflections | 42029 | |
<I/σ(I)> | 13.7 | 1.5 |
Completeness [%] | 99.7 | 97.6 |
Redundancy | 10.8 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1 M calcium acetate, 0.1 M HEPES-NaOH, pH 7.0, 6 % PEG 6000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | calcium acetate | ||
2 | 1 | 1 | HEPES-NaOH | ||
3 | 1 | 1 | PEG 6000 | ||
4 | 1 | 1 | H2O | ||
5 | 1 | 2 | calcium acetate | ||
6 | 1 | 2 | PEG 6000 |