2OZB
Structure of a human Prp31-15.5K-U4 snRNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-03 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.05 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 83.555, 99.896, 169.645 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.600 |
| R-factor | 0.20784 |
| Rwork | 0.206 |
| R-free | 0.24756 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e7k |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.501 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.107 | 0.663 |
| Number of reflections | 42029 | |
| <I/σ(I)> | 13.7 | 1.5 |
| Completeness [%] | 99.7 | 97.6 |
| Redundancy | 10.8 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1 M calcium acetate, 0.1 M HEPES-NaOH, pH 7.0, 6 % PEG 6000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | calcium acetate | ||
| 2 | 1 | 1 | HEPES-NaOH | ||
| 3 | 1 | 1 | PEG 6000 | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | calcium acetate | ||
| 6 | 1 | 2 | PEG 6000 |
