2OZ8
Crystal structure of putative mandelate racemase from Mesorhizobium loti
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-02 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 160.176, 160.176, 82.560 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.480 |
| R-factor | 0.177 |
| Rwork | 0.174 |
| R-free | 0.22900 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.710 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.867 | 2.610 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Rmerge | 0.182 | 0.872 |
| Total number of observations | 75180 | |
| Number of reflections | 38635 | |
| <I/σ(I)> | 15.3 | 3.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.5 | 13.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 294 | 100mM Bis-Tris pH 5.5, 1% PEG 3350, 1M Ammonium sulfate, VAPOR DIFFUSION, temperature 294K |
