2OZ4
Structural Plasticity in IgSF Domain 4 of ICAM-1 Mediates Cell Surface Dimerization
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-01-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 185.396, 69.342, 88.173 |
| Unit cell angles | 90.00, 112.76, 90.00 |
Refinement procedure
| Resolution | 28.500 - 2.700 |
| R-factor | 0.209 |
| Rwork | 0.204 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdb entries 1F11 1p53 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.903 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.087 | 0.543 |
| Number of reflections | 26015 | |
| <I/σ(I)> | 11.1 | 1.2 |
| Completeness [%] | 90.6 | 58.1 |
| Redundancy | 3.5 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 1.5M LITHIUM SULFATE, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
