2OYY
HTHP: a hexameric tyrosine-coordinated heme protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-12-12 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5817 |
| Spacegroup name | H 3 |
| Unit cell lengths | 173.710, 173.710, 45.880 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 86.710 - 2.500 |
| R-factor | 0.23599 |
| Rwork | 0.234 |
| R-free | 0.26867 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.317 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.710 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 35356 | |
| <I/σ(I)> | 3.8 | |
| Completeness [%] | 98.1 | 98.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | 19-20 % PEG 3350, 0.1-0.5 M NH4I, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
