2OYS
Crystal Structure of SP1951 protein from Streptococcus pneumoniae in complex with FMN, Northeast Structural Genomics Target SpR27
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-07-24 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97900 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 76.707, 102.792, 62.158 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.750 - 2.000 |
R-factor | 0.208 |
Rwork | 0.208 |
R-free | 0.22500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1sqs |
RMSD bond length | 0.006 |
RMSD bond angle | 1.000 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | COMO |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.750 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.092 | 0.262 |
Number of reflections | 33663 | |
<I/σ(I)> | 24.77 | 12.23 |
Completeness [%] | 94.7 | 100 |
Redundancy | 7.9 | 8.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 10 mM Tris-HCl pH 7.5, 20% PEG 8000, 50 mM NaCl, 5 mM DTT, 10 mM FMN, VAPOR DIFFUSION, SITTING DROP, temperature 277K |