2OYR
Crystal Structure of UPF0341 Protein (yhiQ) from Shigella flexneri in complex with S-Adenosyl Homocysteine, Northeast Structural Genomics Target SfR275
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-02-18 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97911 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.403, 74.737, 79.423 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.410 - 2.000 |
R-factor | 0.213 |
Rwork | 0.213 |
R-free | 0.23000 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SnB |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.410 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.080 | 0.258 |
Number of reflections | 32699 | |
<I/σ(I)> | 19.81 | 8.51 |
Completeness [%] | 98.3 | 100 |
Redundancy | 6 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH UNDER OIL | 8.5 | 291 | 100mM Tris-HCl pH 8.5, 8% PEG 8000, 50 mM NaCl, 5 mM DTT, 5 mM SAM, MICROBATCH UNDER OIL, temperature 291K |