2OYQ
Crystal structure of RB69 gp43 in complex with DNA with 5-NIMP opposite an abasic site analog
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-08-03 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.99187 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 132.590, 123.478, 164.331 |
Unit cell angles | 90.00, 96.22, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.860 |
R-factor | 0.23416 |
Rwork | 0.228 |
R-free | 0.29420 |
Structure solution method | Isomorphous Replacement |
Starting model (for MR) | 1RV2 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.101 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.960 |
High resolution limit [Å] | 2.860 | 2.860 |
Rmerge | 0.087 | 0.609 |
Number of reflections | 121231 | |
<I/σ(I)> | 10.7 | 2 |
Completeness [%] | 99.6 | 97.3 |
Redundancy | 4.4 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG 2000 MME, sodium acetate, magnesium sulfate, beta-mercaptoethanol, HEPES 7.5, glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 2000 | ||
10 | 1 | 2 | HEPES | ||
2 | 1 | 1 | sodium acetate | ||
3 | 1 | 1 | magnesium sulfate | ||
4 | 1 | 1 | beta-mercaptoethanol | ||
5 | 1 | 1 | HEPES | ||
6 | 1 | 1 | glycerol | ||
7 | 1 | 2 | PEG 2000 | ||
8 | 1 | 2 | sodium acetate | ||
9 | 1 | 2 | magnesium sulfate |