2OYF
Crystal Structure of the complex of phospholipase A2 with indole acetic acid at 1.2 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2006-10-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.90 |
| Spacegroup name | P 43 |
| Unit cell lengths | 52.310, 52.310, 47.835 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.200 |
| R-factor | 0.18863 |
| Rwork | 0.188 |
| R-free | 0.20756 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sxk |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.418 |
| Data reduction software | AUTOMAR |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.230 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Number of reflections | 40258 | |
| <I/σ(I)> | 50.6 | 4.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 24.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 300 | 0.2M ammonium sulphate, 30% PEG 4000, pH 6.8, VAPOR DIFFUSION, SITTING DROP, temperature 300K |
