2OYA
Crystal structure analysis of the dimeric form of the SRCR domain of mouse MARCO
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I711 |
Synchrotron site | MAX II |
Beamline | I711 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-02-10 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 1.09700 |
Spacegroup name | P 1 |
Unit cell lengths | 31.087, 36.553, 44.605 |
Unit cell angles | 116.30, 90.35, 96.41 |
Refinement procedure
Resolution | 14.912 - 1.770 |
R-factor | 0.154 |
Rwork | 0.150 |
R-free | 0.19100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2oy3 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.248 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 14.912 | 1.860 |
High resolution limit [Å] | 1.770 | 1.770 |
Rmerge | 0.037 | 0.074 |
Total number of observations | 7835 | |
Number of reflections | 16364 | |
<I/σ(I)> | 13.8 | 9.1 |
Completeness [%] | 95.4 | 89.4 |
Redundancy | 3.5 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 7% PEG 4000, 0.1M Bis-Tris, 0.1M Lithium sulfate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |