2OY8
The crystal structure of OspA mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-24 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.234, 76.645, 120.348 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.21649 |
| Rwork | 0.214 |
| R-free | 0.25456 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hkd |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.773 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.096 | 0.304 |
| Number of reflections | 24496 | |
| <I/σ(I)> | 20.35 | 7.02 |
| Completeness [%] | 98.6 | 98.8 |
| Redundancy | 7.4 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 31% PEG400, 0.1M Tris-HCl, 2% MPD, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
