2OY5
The crystal structure of OspA mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-11-23 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 37.004, 57.602, 67.746 |
Unit cell angles | 90.00, 95.43, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.800 |
R-factor | 0.18723 |
Rwork | 0.185 |
R-free | 0.22365 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2g8c |
RMSD bond length | 0.017 |
RMSD bond angle | 1.365 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.067 | 0.623 |
Number of reflections | 28857 | |
<I/σ(I)> | 24.26 | 2.37 |
Completeness [%] | 99.8 | 98.1 |
Redundancy | 5.4 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 18% PEG4000, 8.5% isopropanol, 0.1M HEPES, 15% Glycerol, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |