2OXP
Crystal Structure of Staphylococcal Nuclease mutant V66D/P117G/H124L/S128A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-06-09 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 |
| Unit cell lengths | 47.579, 47.579, 62.791 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| Rwork | 0.207 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 9140 |
| Completeness [%] | 96.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 36-39% MPD, 25 mM potassium phosphate, 10% glycerol, 2 meq pdTp, 2 meq Ca2+, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
