2OXO
Crystallization and structure determination of the core-binding domain of bacteriophage lambda integrase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 93 |
Detector technology | IMAGE PLATE |
Collection date | 2006-05-21 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 46.693, 46.693, 111.533 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.070 - 2.000 |
R-factor | 0.23426 |
Rwork | 0.228 |
R-free | 0.28878 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.020 |
RMSD bond angle | 1.623 |
Data reduction software | CrystalClear |
Data scaling software | d*TREK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.073 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.035 | 0.174 |
Number of reflections | 8503 | |
<I/σ(I)> | 34.2 | 7.9 |
Completeness [%] | 74.5 | |
Redundancy | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.1 M Tris, 0.2 M lithium sulfate, 30% PEG 4000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |