2OX7

Crystal structure of protein EF1440 from Enterococcus faecalis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2007-02-18
Detector	MAR CCD 165 mm
Wavelength(s)	0.97961
Spacegroup name	P 21 21 21
Unit cell lengths	64.346, 70.376, 150.914
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.630 - 1.777
R-factor	0.19208
Rwork	0.190
R-free	0.23531
Structure solution method	SAD
RMSD bond length	0.017
RMSD bond angle	1.532
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELX
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.474	25.470	1.870
High resolution limit [Å]	1.777	5.620	1.777
Rmerge	0.080	0.055	0.383
Total number of observations		30071	116843
Number of reflections	66447
<I/σ(I)>	6.2	11.2	2
Completeness [%]	99.4	98.5	96.1
Redundancy	14.4	13.1	12.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	2.0M SODIUM CHLORIDE, 10% PEG 6000, pH 7.50, VAPOR DIFFUSION, SITTING DROP, temperature 295K