2OX6
Crystal structure of gene product SO3848 from Shewanella oneidensis MR-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97980 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.068, 88.680, 117.527 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.710 - 1.700 |
| R-factor | 0.20105 |
| Rwork | 0.199 |
| R-free | 0.23930 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.193 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.710 | 1.746 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.062 | 0.581 |
| Number of reflections | 71755 | |
| <I/σ(I)> | 26.25 | 1.2 |
| Completeness [%] | 94.7 | 56.42 |
| Redundancy | 4.6 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 1.0M (NH4)2SO4, 0.1M Bis-Tris pH 5.5, 1% PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
