2OX5
The SoxYZ complex of Paracoccus pantotrophus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-03 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9793, 0.8731 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 206.605, 54.708, 77.884 |
| Unit cell angles | 90.00, 98.58, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.980 |
| R-factor | 0.175 |
| Rwork | 0.173 |
| R-free | 0.21100 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.354 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 102.147 | 39.910 | 2.090 |
| High resolution limit [Å] | 1.981 | 6.260 | 1.980 |
| Rmerge | 0.070 | 0.044 | 0.270 |
| Total number of observations | 7868 | 23487 | |
| Number of reflections | 59443 | ||
| <I/σ(I)> | 8.6 | 11.5 | 2.7 |
| Completeness [%] | 98.7 | 99.6 | 91.3 |
| Redundancy | 4 | 3.9 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 2 microl of protein at 10 mg/ml in 10 mM Tris-HCl pH 8.0, 5 mM beta-mercaptoethanol and 200 mM NaCl mixed with 2 microl of 28-30 % (w/v) PEG 3350, 100 mM sodium acetate pH 4.75, 200 mM NH4SO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
