2OX3
R-state, PEP and Fru-6-P-bound, Escherichia coli fructose-1,6-bisphosphatase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-09-13 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 44.687, 83.112, 174.422 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 75.030 - 2.180 |
| R-factor | 0.216 |
| Rwork | 0.212 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID: 2GQ1 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.030 | 2.320 |
| High resolution limit [Å] | 2.180 | 2.180 |
| Rmerge | 0.046 | 0.238 |
| Number of reflections | 17261 | |
| <I/σ(I)> | 21.1 | 6.8 |
| Completeness [%] | 98.7 | 92.8 |
| Redundancy | 6.7 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 50 mM sodium acetate, pH 5.6, 16% (w/v) PEG 1500, and 20% (w/v) sucrose, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
