2OWX
THERMUS THERMOPHILUS AMYLOMALTASE AT pH 5.6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-01-22 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.830 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 93.801, 93.801, 154.769 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.920 - 2.500 |
| R-factor | 0.22295 |
| Rwork | 0.220 |
| R-free | 0.27641 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cwy |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.573 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.079 | 0.422 |
| Number of reflections | 25247 | |
| <I/σ(I)> | 9 | 1.4 |
| Completeness [%] | 86.9 | 92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | SODIUM MALONATE, PH 5.6, 1 mM DTT, pH 5.60, VAPOR DIFFUSION, HANGING DROP |
