2OWM

Motor domain of Neurospora crassa kinesin-3 (NcKin3)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	100
Detector technology	CCD
Collection date	2004-05-07
Detector	MAR CCD 165 mm
Spacegroup name	P 1 21 1
Unit cell lengths	75.945, 98.408, 111.764
Unit cell angles	90.00, 91.86, 90.00

Refinement procedure

Resolution	111.800 - 3.250
R-factor	0.213
Rwork	0.210
R-free	0.25600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	TRUNCATED MODEL DERIVED FROM ADP COMPLEX OF KIF1A (1I5S)
RMSD bond length	0.018
RMSD bond angle	1.844
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (refmac_5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	111.800	3.310
High resolution limit [Å]	3.250	3.250
Rmerge	0.143	0.705
Number of reflections	26100
<I/σ(I)>	6
Completeness [%]	100.0	100
Redundancy	3.8	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5	293	1.5ul protein solution (50mM phosphate buffer pH 7.5, 150mM sodium chloride, 50uM ATP, 1mM magnesium chloride, EGTA, DTT) mixed with 0.5ul reservoir (0.1M Hepes pH 7.5, 0.2M sodium potassium tartrate, 24% PEG3350) and 0.2ul 0.1M magnesium chloride , VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 7.50