2OWJ
Structure of an early-microsecond photolyzed state of CO-bjFixLH, dark state
Experimental procedure
Experimental method | LAUE |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-ID-B |
Synchrotron site | APS |
Beamline | 14-ID-B |
Temperature [K] | 287 |
Detector technology | CCD |
Collection date | 2002-04-17 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.8-1.5 |
Spacegroup name | H 3 2 |
Unit cell lengths | 128.500, 128.500, 58.400 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.100 - 2.500 |
R-factor | 0.216 |
Rwork | 0.213 |
R-free | 0.29100 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.016 |
RMSD bond angle | 1.608 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.100 | 2.550 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 6352 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 4M NaCl, 50mM CAPSO, 1% PEI, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 4M NaCl, 50mM CAPSO, 1% PEI, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 4M NaCl, 50mM CAPSO, 1% PEI, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |