2OVW
ENDOGLUCANASE I COMPLEXED WITH CELLOBIOSE
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU FR-C |
Temperature [K] | 120 |
Detector technology | IMAGE PLATE |
Collection date | 1995-08-23 |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 67.930, 78.310, 142.470 |
Unit cell angles | 90.00, 96.98, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.300 |
R-factor | 0.209 * |
Rwork | 0.209 |
R-free | 0.28000 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | NATIVE STRUCTURE (2 MOLECULES IN ASYMMETRIC UNIT) |
RMSD bond length | 0.013 |
RMSD bond angle | 0.037 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.400 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.077 | 0.270 |
Number of reflections | 57194 | |
<I/σ(I)> | 9.6 | 2.29 |
Completeness [%] | 86.6 | 54 * |
Redundancy | 1.9 | 1.37 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 * | 16 * | 22 % PEG 8K, 0.2 M MAGNESIUM CHLORIDE, PH 6.5 FOR 0.1 M MOPS. METHOD: HANGING DROP VAPOR DIFFUSION THE NATIVE CRYSTALS WERE SOAKED FOR 1 HOUR IN STABILIZING SOLUTION CONTAINING 20 MM OF CELLOBIOSE., vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG8000 | 20-26 (%(w/v)) | |
2 | 1 | reservoir | 0.15-0.3 (M) | ||
3 | 1 | reservoir | MOPS | 0.1 (M) | pH6.4 |