2OVI
Structure of the Heme Binding Protein ChuX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-04-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97950 |
Spacegroup name | P 41 |
Unit cell lengths | 76.560, 76.560, 140.864 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.050 |
Rwork | 0.213 |
R-free | 0.25000 |
Structure solution method | SAD |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE (2.06) |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.120 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.055 | 0.950 |
Number of reflections | 50833 | |
<I/σ(I)> | 15.1 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.1 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 40 mg/ml protein, equal amount of protein solution and reservoir solution consisting of 1.50 M Ammonium sulfate, 50mM Hepes, pH 7.5, 2% (v/v) PEG 400, crystal appeared after 2-4 days, cryoprotection: Paratone-N oil, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 40 mg/ml protein, equal amount of protein solution and reservoir solution consisting of 1.50 M Ammonium sulfate, 50mM Hepes, pH 7.5, 2% (v/v) PEG 400, crystal appeared after 2-4 days, cryoprotection: Paratone-N oil, VAPOR DIFFUSION, HANGING DROP, temperature 295K |