2OVF

Crystal Structure of StaL-PAP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X8C
Synchrotron site	NSLS
Beamline	X8C
Temperature [K]	100
Detector technology	CCD
Collection date	2005-11-20
Detector	ADSC QUANTUM 4
Wavelength(s)	1.1
Spacegroup name	P 61 2 2
Unit cell lengths	87.392, 87.392, 169.377
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	76.920 - 2.950
R-factor	0.226
Rwork	0.224
R-free	0.26900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ov8
RMSD bond length	0.013
RMSD bond angle	1.614
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.060
High resolution limit [Å]	2.950	6.350	2.950
Rmerge	0.048	0.025	0.532
Number of reflections	8661
<I/σ(I)>	17.4
Completeness [%]	99.9	98.9	100
Redundancy	11.2	9.8	11.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	0.1M Bis-Tris propane pH6.5,0.2M NaI, 18% PEG 3350, 10mM PAP, 5mM desulfo-A47934, vapor diffusion, hanging drop, temperature 295K