2OVC

Crystal structure of a coiled-coil tetramerization domain from Kv7.4 channels

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-05-20
Detector	ADSC QUANTUM 210
Wavelength(s)	1.116
Spacegroup name	I 4
Unit cell lengths	33.185, 33.185, 55.283
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.460 - 2.070
R-factor	0.197
Rwork	0.196
R-free	0.22400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.586
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.460	50.000	2.140
High resolution limit [Å]	2.070	4.460	2.070
Rmerge	0.053	0.043	0.096
Number of reflections	1841
<I/σ(I)>	15.3
Completeness [%]	99.6	95.9	100
Redundancy	7.3	7.1	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	289	45% MPD, 0.2M ammonium acetate, 0.1M Bis-Tris, pH 5.5, vapor diffusion, hanging drop, temperature 289K
1	VAPOR DIFFUSION, HANGING DROP	5.5	289	45% MPD, 0.2M ammonium acetate, 0.1M Bis-Tris, pH 5.5, vapor diffusion, hanging drop, temperature 289K