2OVB
Crystal Structure of StaL-sulfate complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-10-02 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 88.733, 88.733, 169.691 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 76.920 - 2.610 |
R-factor | 0.23 |
Rwork | 0.228 |
R-free | 0.26900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ov8 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.193 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.700 |
High resolution limit [Å] | 2.610 | 5.620 | 2.610 |
Rmerge | 0.051 | 0.038 | 0.325 |
Number of reflections | 12609 | ||
<I/σ(I)> | 22.4 | ||
Completeness [%] | 99.3 | 99.6 | 93.4 |
Redundancy | 10.5 | 9.7 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 0.1M Bis-Tris pH5.5,0.2M Ammonium Sulfate, 22% PEG 3350, vapor diffusion, hanging drop, temperature 295K |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 0.1M Bis-Tris pH5.5,0.2M Ammonium Sulfate, 22% PEG 3350, vapor diffusion, hanging drop, temperature 295K |