2OVA

X-ray structure of Human Complement Protein C8gamma Y83W Mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2004-03-14
Detector	ADSC QUANTUM 315
Wavelength(s)	0.96972
Spacegroup name	P 21 21 21
Unit cell lengths	42.256, 58.938, 71.996
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	500.000 - 1.500
Rwork	0.219
R-free	0.22500
RMSD bond length	0.005
RMSD bond angle	1.119
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	500.000	1.350
High resolution limit [Å]	1.300	1.300
Rmerge	0.053	0.144
Number of reflections	42289
<I/σ(I)>	26.7	2.6
Completeness [%]	94.2	75.1
Redundancy	8.4	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	290	0.1 M sodium citrate and 24-26% PEG 4000, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K