2OV8
Crystal Structure of StaL
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-06-09 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9795, 0.9794, 0.9643 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 86.018, 86.018, 164.753 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 74.540 - 2.580 |
R-factor | 0.251 |
Rwork | 0.249 |
R-free | 0.27900 |
Structure solution method | MAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.480 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE (2.09) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.670 |
High resolution limit [Å] | 2.580 | 2.580 |
Rmerge | 0.055 | 0.471 |
Number of reflections | 12023 | |
<I/σ(I)> | 20.7 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 29.7 | 22.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 295 | 0.1M NaAc pH4.6, 3.0M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 295 | 0.1M NaAc pH4.6, 3.0M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 295 | 0.1M NaAc pH4.6, 3.0M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 295 | 0.1M NaAc pH4.6, 3.0M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 295K |