2OV7
The first domain of the ribosomal protein L1 from Thermus thermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-03-19 |
Detector | MAR CCD 165 mm |
Spacegroup name | P 31 |
Unit cell lengths | 79.200, 79.200, 47.640 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 15.000 - 2.300 |
Rwork | 0.193 |
R-free | 0.23280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | The first domain of L1 from Thermus thermophilus |
RMSD bond length | 0.006 |
RMSD bond angle | 0.804 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.400 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.149 | 0.327 |
Number of reflections | 14608 | |
<I/σ(I)> | 7.9 | 3.2 |
Completeness [%] | 96.8 | 98.4 |
Redundancy | 4.6 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 277 | 30% v/v polyethylenglycol 4K, 100 mM Tris-HCl, 200 mM LiSO4, pH 8.5, VAPOR DIFFUSION, temperature 277K |